mouse th1 th2 10-plex elisa bead assay Search Results


mouse th1/th2 cytokine 10plex flowcytomix multiplex  (Bender MedSystems)
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Mouse Th1/Th2 Cytokine 10plex Flowcytomix Multiplex, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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th1/th2 human 10-plex kit  (Meso Scale Diagnostics LLC)
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Th1/Th2 Human 10 Plex Kit, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luminextm cytokine mouse magnetic or mesoscale© 10-plex panel  (Meso Scale Diagnostics LLC)
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Luminextm Cytokine Mouse Magnetic Or Mesoscale© 10 Plex Panel, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10-plex multiplex elisa proinflammatory panel (mouse) kit  (Meso Scale Diagnostics LLC)
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10 Plex Multiplex Elisa Proinflammatory Panel (Mouse) Kit, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytometric bead array mouse th1/th2 cytokine kit  (Becton Dickinson)
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Becton Dickinson cytometric bead array mouse th1/th2 cytokine kit
Representative experiment of <t> cytokine </t> production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.
Cytometric Bead Array Mouse Th1/Th2 Cytokine Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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luminex 200 system  (DiaSorin Biotechnology)
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DiaSorin Biotechnology luminex 200 system
Representative experiment of <t> cytokine </t> production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.
Luminex 200 System, supplied by DiaSorin Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cytoflex flow cytometers  (Danaher Inc)
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Representative experiment of <t> cytokine </t> production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.
Cytoflex Flow Cytometers, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facscanto flow cytometer  (Becton Dickinson)
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Representative experiment of <t> cytokine </t> production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.
Facscanto Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-collagen enzyme-linked immunosorbent assay (elisa)  (Bender MedSystems)
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Bender MedSystems anti-collagen enzyme-linked immunosorbent assay (elisa)
Diminished induction of Th17-related cytokines in myeloid pten -/- mice after induction of collagen-induced arthritis (CIA). Serum samples of wt (n = 16) and myeloid pten -/- (n = 9) mice obtained 4 weeks after induction of CIA. Levels of the indicated cytokines were analyzed by <t>ELISA.</t> Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. ELISA enzyme-linked <t>immunosorbent</t> assay, Th T helper, wt wild-type
Anti Collagen Enzyme Linked Immunosorbent Assay (Elisa), supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facscalibur™ flow cytometer  (Becton Dickinson)
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Becton Dickinson facscalibur™ flow cytometer
Diminished induction of Th17-related cytokines in myeloid pten -/- mice after induction of collagen-induced arthritis (CIA). Serum samples of wt (n = 16) and myeloid pten -/- (n = 9) mice obtained 4 weeks after induction of CIA. Levels of the indicated cytokines were analyzed by <t>ELISA.</t> Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. ELISA enzyme-linked <t>immunosorbent</t> assay, Th T helper, wt wild-type
Facscalibur™ Flow Cytometer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sodium chloride 99.9% (nacl)  (Applichem inc)
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Diminished induction of Th17-related cytokines in myeloid pten -/- mice after induction of collagen-induced arthritis (CIA). Serum samples of wt (n = 16) and myeloid pten -/- (n = 9) mice obtained 4 weeks after induction of CIA. Levels of the indicated cytokines were analyzed by <t>ELISA.</t> Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. ELISA enzyme-linked <t>immunosorbent</t> assay, Th T helper, wt wild-type
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Image Search Results


Representative experiment of cytokine production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.

Journal: Bio-protocol

Article Title: Detection of Antigen-specific T cells in Spleens of Vaccinated Mice Applying 3 [H]-Thymidine Incorporation Assay and Luminex Multiple Cytokine Analysis Technology

doi: 10.21769/BioProtoc.3252

Figure Lengend Snippet: Representative experiment of cytokine production. Spleen cells obtained from non-immunized and immunized mice were treated with either complete medium, or 5 μg/ml N- Li EF-2. Data are means ± S.D. from three replicates for each condition.

Article Snippet: Note: In the case of not having a Luminex multi-analyte profiling system, a cytometric bead array that it is compatible with flow cytometers can be used as an alternative, i.e., Cytometric Bead Array Mouse Th1/Th2 Cytokine Kit (BD Biosciences), LEGENDplex Mouse Th1/Th2 Panel (8-plex) (Biolegend), FlowCytomix mouse Th1/Th2 10plex (Bender MedSystems).

Techniques:

Diminished induction of Th17-related cytokines in myeloid pten -/- mice after induction of collagen-induced arthritis (CIA). Serum samples of wt (n = 16) and myeloid pten -/- (n = 9) mice obtained 4 weeks after induction of CIA. Levels of the indicated cytokines were analyzed by ELISA. Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. ELISA enzyme-linked immunosorbent assay, Th T helper, wt wild-type

Journal: Arthritis Research & Therapy

Article Title: Phosphatase and tensin homolog (PTEN) in antigen-presenting cells controls Th17-mediated autoimmune arthritis

doi: 10.1186/s13075-015-0742-y

Figure Lengend Snippet: Diminished induction of Th17-related cytokines in myeloid pten -/- mice after induction of collagen-induced arthritis (CIA). Serum samples of wt (n = 16) and myeloid pten -/- (n = 9) mice obtained 4 weeks after induction of CIA. Levels of the indicated cytokines were analyzed by ELISA. Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. ELISA enzyme-linked immunosorbent assay, Th T helper, wt wild-type

Article Snippet: Anti-collagen enzyme-linked immunosorbent assay (ELISA), Mouse Th1/Th2 10plex FlowCytomix Multiplex as well as IL-23 and IL-22 FlowCytomix simplex were from Bender MedSystems (Vienna, Austria) and used according to the manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay

PTEN in APCs allows for priming of Th17 cells in vivo. a , b Cells of draining LN of wt (n = 4) and myeloid pten -/- (n = 6) mice were harvested 2 weeks after induction of CIA. a mRNA levels of the indicated cytokines were determined by qPCR. Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05. b LN cells of wt and myeloid pten -/- mice were stimulated in vitro with anti-CD3 for 3 days and levels of the indicated cytokines in the supernatant were measured by ELISA. Horizontal bars are means of each group ± s.d. Each data point represents an individual animal * P ≤ 0.05. APCs antigen-presenting cells, CIA collagen-induced arthritis, ELISA enzyme-linked immunosorbent assay, LN lymph node, PTEN phosphatase and tensin homolog, qPCR quantitative real-time polymerase chain reaction, Th T helper, wt wild-type

Journal: Arthritis Research & Therapy

Article Title: Phosphatase and tensin homolog (PTEN) in antigen-presenting cells controls Th17-mediated autoimmune arthritis

doi: 10.1186/s13075-015-0742-y

Figure Lengend Snippet: PTEN in APCs allows for priming of Th17 cells in vivo. a , b Cells of draining LN of wt (n = 4) and myeloid pten -/- (n = 6) mice were harvested 2 weeks after induction of CIA. a mRNA levels of the indicated cytokines were determined by qPCR. Horizontal bars are means of each group ± s.e. Each data point represents an individual animal * P ≤ 0.05. b LN cells of wt and myeloid pten -/- mice were stimulated in vitro with anti-CD3 for 3 days and levels of the indicated cytokines in the supernatant were measured by ELISA. Horizontal bars are means of each group ± s.d. Each data point represents an individual animal * P ≤ 0.05. APCs antigen-presenting cells, CIA collagen-induced arthritis, ELISA enzyme-linked immunosorbent assay, LN lymph node, PTEN phosphatase and tensin homolog, qPCR quantitative real-time polymerase chain reaction, Th T helper, wt wild-type

Article Snippet: Anti-collagen enzyme-linked immunosorbent assay (ELISA), Mouse Th1/Th2 10plex FlowCytomix Multiplex as well as IL-23 and IL-22 FlowCytomix simplex were from Bender MedSystems (Vienna, Austria) and used according to the manufacturer’s protocols.

Techniques: In Vivo, In Vitro, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction